FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1702707
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83790.4920987580364678No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG25590.15029009688689832TruSeq Adapter, Index 8 (96% over 29bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17210.10107434808220087No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA8600.031.3055651
ATGCCGT12650.029.99927144
CCGTCTT12300.029.17002547
GTATGCC14000.027.3529142
TATGCCG14950.025.84552443
ATGAATG6150.025.80425369
TGCCGTC13950.024.9778945
CTTGAAA14500.024.50630457
GTCTTCT12000.024.43690949
TGCTTGA14300.024.12528855
CTCGTAT15150.023.9103139
TCTCGTA15650.023.36753338
GATCTTT92100.023.299116
ATCTTTT88850.023.2971297
GACTAGT1352.6614362E-722.99944339
TCGTATG16200.022.57352640
GCCGTCT15500.022.48010346
ATCTGGC13050.022.4707187
TCTTTTT91950.022.436658
CGTATGC17200.022.26399441
Produced by FastQC (version 0.11.2)