FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433226
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA102780.7171234683155343No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG32610.22752866610011263TruSeq Adapter, Index 8 (96% over 29bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30270.21120186209292882No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19210.13403329272564132No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG17840.12447443738810209TruSeq Adapter, Index 8 (96% over 29bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17620.12293943872076002No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG15130.10556604471311573TruSeq Adapter, Index 8 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT17150.031.9851547
GTATGCC20000.030.53223842
GTCTTCT16950.030.12363449
CTTGAAA19150.030.08590157
TATGCCG19850.029.8939543
TGCTTGA18400.029.81224355
GCCGTCT18400.029.62474446
GTGTAAC703.8418213E-529.5825271
CTCGTAT18900.029.57117339
TCTCGTA18700.029.51846138
TTTCGGA6550.028.9803371
CGTCTTC17700.028.65229648
CGTATGC21400.028.53480141
TCTTCTG17950.028.06103950
TCGTATG20650.027.73341640
ATGCCGT21300.027.69694544
GCTTGAA20950.027.50095756
TGCCGTC21150.027.240945
AATCTCG20800.027.03582436
CTTCTGC19300.025.91946651
Produced by FastQC (version 0.11.2)