FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010204
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40500.4009091233057877No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG20250.20045456165289385TruSeq Adapter, Index 8 (96% over 29bp)
GTCCTGGAGACAGTGGGGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCT12860.12730102038796123No Hit
GTCCTGAAGGAACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCCTCATTGTGCGCAAGCATTTTGAG12380.12254950485248524No Hit
GTATAGACCGTACCCGAAACCGACACAGGTGGTCAGGTAGAGTATACCAAGGCGCTTGAGAGAAGTGTCCTGAAG11430.11314546368852232No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG11160.11047273619981705TruSeq Adapter, Index 8 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG10820.10710707936218823TruSeq Adapter, Index 8 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGAATG2050.028.6095169
CTATAGG756.144471E-527.612061
TTTCGGA4200.027.118991
TACATAT1359.424184E-925.55533668
TATCGTA951.0506737E-525.42083438
ATCTTTT35100.024.57243712
CCGTCTT9700.024.18535847
GGTTTGT1001.5635233E-524.14979218
CGCGTAT1301.8470746E-723.88440939
ATGAATA2750.023.83615769
GTATGCC10900.023.73832942
GTCTTCT9050.023.63515749
GATCTTT38350.023.2996711
TCTTTTT37650.023.18306713
GCGTATG1352.6588168E-722.99980240
TATGCCG11150.022.5872543
CTTTTTT38800.022.4959414
ATGCCGT11300.021.98211144
CTTGAAA11350.021.58131257
CGTCTTC10500.021.35695848
Produced by FastQC (version 0.11.2)