FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483568
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG55650.37510919620806055RNA PCR Primer, Index 46 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51550.3474731188593984No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG31740.21394368171866746RNA PCR Primer, Index 46 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG24450.16480538809141204RNA PCR Primer, Index 46 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG16670.11236424619565805RNA PCR Primer, Index 46 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC27600.028.12453542
ATGCCGT28400.027.57524744
GTCTTCT24700.027.2363949
TATGCCG29300.026.72822643
TGCCGTC27000.026.70511245
GCCGTCT26800.026.13202946
TCGTATG29000.025.69612340
CGTATGC29850.025.42671241
CGTCTTC26650.025.37293648
TGCTTGA29250.025.35855155
TTGAAAA30900.025.12094158
TTTCGGA5950.024.9432871
GCTTGAA30000.024.72458856
CTCGTAT28500.024.57327739
TCTGCTT27100.024.56970253
AATCTCG28750.024.47959136
TTCTGCT26850.024.41300452
TGAAAAA34050.024.31677659
CTGCTTG28950.024.31047654
CGAATCT30550.023.60190234
Produced by FastQC (version 0.11.2)