FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1325617
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63360.4779661093664309No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG42320.31924756547328526TruSeq Adapter, Index 3 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG21900.16520608893820762TruSeq Adapter, Index 3 (96% over 28bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16260.12265986329384732No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG16240.12250899015326447TruSeq Adapter, Index 3 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA6250.033.1347271
GTCTTCT16500.025.29979149
CCGTCTT18050.024.65630747
GTATGCC18750.024.655842
ATGCCGT18800.024.40671544
TATGCCG18900.024.27757843
GATCTTT72500.024.1735946
AGGAATG7600.024.05901369
GCTTGAA19300.023.9531756
ATCTTTT71450.023.80456212
TTCTGCT17700.023.58455352
TGCCGTC18800.023.4891745
TGCTTGA19250.023.4777355
CTTCTGC17950.023.44827551
CTTGAAA19750.023.40740257
GCCGTCT19500.022.8228946
TCTTCTG18300.022.81128950
TCTTTTT75150.022.72436513
TCGTATG19300.022.5231340
CTGCTTG19100.022.39772254
Produced by FastQC (version 0.11.2)