FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483560
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34340.23147024724311788No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG24770.16696325055946507TruSeq Adapter, Index 3 (96% over 28bp)
GTCCTGGAGACAGTGGGGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCT19030.12827253363530966No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGAATG6550.028.44219269
ATGCCGT11300.026.25612444
TATGCCG11550.025.98650643
GTATGCC11900.025.22219742
CCGTCTT11250.024.53285247
ATCTTTT41050.024.28983512
TTTCGGA6850.024.1877541
TCTAGGG2650.023.4342942
GTCTTCT11000.023.20863749
ATCTGTG6000.022.999557
CTTGAAA11750.022.90168257
TCTTTTT44300.022.42996613
GCCGTCT12500.022.35556446
GATCTTT46450.022.20881511
TCTTCTG11850.022.12615250
CTTTTTT45650.021.7666514
ATCTGGG8800.021.5620787
ATAGTGT1154.604484E-520.999598
GATCTGG27450.020.988666
CGATCTG88250.020.7973275
Produced by FastQC (version 0.11.2)