FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1537175
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATCCCCATCACGACAGAGTTTCACAAGATTACCCATGCCTTCCGGCAAAGGTGGTAGACTCGCTGGCTCT17740.11540650869289444No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGAATG4800.032.3458469
ATATGGC450.004622446430.6676523
ATCTGTG3200.028.0303237
ATCTGGG7100.025.752677
GTTTTAT951.0476297E-525.4317971
AATCAGG2700.024.27697836
GAATCAG2650.023.43318735
CTCTAGG902.1207302E-423.0097181
GGTTGCA3200.022.63987716
GGAATCA3000.021.8492834
TCTTTTT14550.021.81513213
GTCCTAG1906.712071E-1021.7986831
CTAGGAC3350.021.6275583
ATATGTG800.002900428421.5617875
GATCTGG19750.021.4853696
ATGGTTG2650.020.83085614
GATCTTT17150.020.7194336
TTCAGTA1004.3347842E-420.7006632
TATATGA1004.3347842E-420.70066314
CTTCCGA164650.020.4802571
Produced by FastQC (version 0.11.2)