FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1469481
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40830.2778532012322718No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG25430.17305429604057487TruSeq Adapter, Index 3 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG15000.10207685570619832TruSeq Adapter, Index 3 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGTT703.852293E-529.570817
GTCTTCT11900.026.67170349
CCGTCTT12650.026.45398347
GTATGCC13350.025.32530642
CTCGTAT12600.024.9161439
ATTAACT1251.2648707E-724.8403194
ATCTTTT45450.024.67112412
TATGCCG14000.024.64233643
ATAGTCT700.00133418824.64233441
CTTGAAA13500.024.53281257
ATGCCGT13800.024.24948944
CTTCTGC13250.023.95420851
TGCCGTC14350.023.8008945
GCCGTCT14650.023.78447746
TCTTTTT48050.023.47976913
CATACGT750.001993136523.0002962
ATAAGGC902.126561E-423.0002943
TCTTCTG13950.022.9995150
TCGTATG15350.022.92459540
TTTCGGA5750.022.8096071
Produced by FastQC (version 0.11.2)