FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667485
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG13460.20165247159112187RNA PCR Primer, Index 33 (96% over 29bp)
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC10380.15550911256432728No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTT7740.1159576619699319No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCA7600.11386023655962306No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA7280.10906612133606No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7210.10801740863090556No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAATCAAGAACGAAAGTTAGAGGTTCGAA7160.10726832812722384No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC7070.10591998322059672No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT6950.10412219001176057No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACGT400.00259569634.4996263
AGACGTA553.2557826E-431.36329867
ATACGTA553.2557826E-431.3632984
ATGCCGT4750.028.32600844
CCGTCTT4700.027.89331447
GGCCTAA500.007733043727.599743
TCTATCG500.007733043727.59978
GTATGCC4950.027.18152442
CTCGTAT5150.026.79582639
AGTCTAA658.6538255E-426.53817464
TACGTAG658.6538255E-426.5381745
GTCTTCT4950.026.48456249
TTTCGGA1705.456968E-1226.3998661
TGCCGTC5000.026.21971545
GCTTGAA5200.025.8747256
GATATAC809.556645E-525.8747246
AATTACT809.556645E-525.8747230
CGTATGC5600.025.25865441
TATGCCG5350.025.1492643
GCAATCT5300.024.7355834
Produced by FastQC (version 0.11.2)