FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320122
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA127910.9689255993006707No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG13570.10279352968892269RNA PCR Primer, Index 46 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT12850.028.72702644
CTTGAAA13200.027.96532457
GTATGCC13550.027.75218842
CCGTCTT12200.027.42975847
GTCTTCT11250.026.68045249
GCTTGAA13550.026.47915356
TGCCGTC13250.026.29756545
GGTCCAA2650.026.03719311
TATGCCG14600.025.75631343
AATCTCG14100.025.69095236
TCTCGTA14150.025.60017238
CTCGTAT14650.025.43291539
TTTCGGA5300.025.3987671
CTTCTGC12050.024.90913851
GCCGTCT13400.024.71590246
GAATCTC14950.024.23026535
TAGGGTA1001.5627676E-524.152244
TGCTTGA14850.023.69737255
ATCTTTT46400.023.42084712
TCGTATG16450.023.2791540
Produced by FastQC (version 0.11.2)