FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294742
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA62470.482489947804273No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG29430.22730397252888995RNA PCR Primer, Index 33 (96% over 29bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20440.15786928978900816No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG13020.10056057500258737RNA PCR Primer, Index 33 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAATG854.4295066E-628.41111418
ATGCCGT10950.027.0952744
TTTCGGA7100.026.7331
GTATGCC11050.026.22564342
GTCTTCT11200.025.87540449
CTCGTAT11000.025.71759239
ATCTTTT62300.025.0299227
GATCTTT64950.024.9647856
CGTATGC11700.024.76866341
TCTTTTT63300.024.41658
CTTCTGC11600.024.38831151
GCCGTCT11900.024.3523846
GGCAATA7550.024.21985869
GCTTGAA12400.023.92779556
TGCCGTC12350.023.4650545
TCGTATG12400.023.37043240
CTTTTTT66450.023.3118789
TGCTTGA12650.023.1821855
ATGCTAC1502.8790964E-823.0065821
CGTCTTC12650.022.90856648
Produced by FastQC (version 0.11.2)