FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112322
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10080.8974199177365076No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG10060.8956393226616335RNA PCR Primer, Index 33 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG4070.36235109773686364RNA PCR Primer, Index 33 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG3630.32317800608963515RNA PCR Primer, Index 33 (96% over 29bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2460.21901319420950482No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG2150.19141397054895745RNA PCR Primer, Index 33 (96% over 29bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1820.1620341518135361No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG1620.14422820106479584RNA PCR Primer, Index 33 (96% over 29bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.10505510941756736No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCA150.00235006368.999562
AGGGAGT150.00235006368.9995624
TATTTAC200.00734203751.74966443
TACCATC200.00734203751.74966459
CCTCCCT200.00734203751.74966428
ATGAGAC200.00734203751.7496646
CAAGCGT200.00734203751.74966441
ATGAAAC200.00734203751.74966430
GCTGTGT200.00734203751.7496643
TATCGTA200.00734203751.74966438
GGATAAG352.253822E-549.3073461
ATCTGAG352.2597716E-549.2853977
ATCTCTC306.287238E-445.9997027
TCTGAGC404.973315E-543.1247188
TCTGAGA350.001342678639.42831813
CTGAGAG350.001342678639.42831814
GGTCCCG350.001342678639.42831848
GGTTGTT350.001342678639.42831824
CGGTCAC350.001342678639.42831816
TGAGAGG350.001342678639.42831815
Produced by FastQC (version 0.11.2)