FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1757782
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG34850.19826121783019737RNA PCR Primer, Index 33 (96% over 29bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30140.17146608623822523No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAATG5200.030.5184969
CTAGTTT500.007734782527.600928
CCGTCTT13000.025.21092647
ATGCCGT13250.024.99561944
TGCCGTC13450.024.62393845
TATGCCG13750.024.58849343
ACTTTAC851.4439621E-424.3523488
GTATGCC13750.024.33759142
ATAGGAT2900.023.7945563
TAGGATC2650.023.4353944
GTCTTCT14200.023.32337649
GGCAATA8150.023.28164369
CTTCTGC14500.023.0787551
TCTGCTT14950.022.84559853
CGTCTTC15050.022.46457148
GCCGTCT14750.022.4536946
TTCTGCT14600.022.44808452
GTATAGT1704.4219632E-922.3325161
TCTGACC4950.022.3024888
ATCTTTT38150.022.15731612
Produced by FastQC (version 0.11.2)