Basic Statistics
Measure | Value |
---|---|
Filename | H32MNAFXY_n01_zt2wtlarvs63.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1847110 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 52 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG | 8343 | 0.4516785681415833 | TruSeq Adapter, Index 10 (96% over 28bp) |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 7811 | 0.42287681838114677 | No Hit |
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG | 3998 | 0.21644623222222825 | TruSeq Adapter, Index 10 (96% over 28bp) |
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG | 2017 | 0.10919761140376046 | RNA PCR Primer, Index 10 (96% over 28bp) |
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG | 2015 | 0.10908933414902199 | TruSeq Adapter, Index 10 (96% over 28bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 2690 | 0.0 | 33.986282 | 45 |
CCGTCTT | 2740 | 0.0 | 33.366093 | 47 |
ATGCCGT | 2785 | 0.0 | 33.074715 | 44 |
CTTGAAA | 2935 | 0.0 | 31.854532 | 57 |
GTCTTCT | 2930 | 0.0 | 31.555656 | 49 |
CTTCTGC | 2965 | 0.0 | 31.299519 | 51 |
GTATGCC | 2945 | 0.0 | 31.277788 | 42 |
TCTTCTG | 2955 | 0.0 | 31.171942 | 50 |
TATGCCG | 2955 | 0.0 | 31.171942 | 43 |
GCCGTCT | 2930 | 0.0 | 31.084677 | 46 |
TGCTTGA | 3000 | 0.0 | 31.049353 | 55 |
TTCTGCT | 3005 | 0.0 | 30.99769 | 52 |
CTGCTTG | 3035 | 0.0 | 30.804962 | 54 |
TCTGCTT | 3030 | 0.0 | 30.741936 | 53 |
CGTCTTC | 3020 | 0.0 | 30.501022 | 48 |
GCTTGAA | 3100 | 0.0 | 30.15905 | 56 |
TCTCGTA | 2830 | 0.0 | 29.135437 | 38 |
CTCGTAT | 3005 | 0.0 | 28.816372 | 39 |
TCGTATG | 3310 | 0.0 | 27.724497 | 40 |
TGAAAAA | 3475 | 0.0 | 27.202312 | 59 |