FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847110
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG83430.4516785681415833TruSeq Adapter, Index 10 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78110.42287681838114677No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG39980.21644623222222825TruSeq Adapter, Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG20170.10919761140376046RNA PCR Primer, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG20150.10908933414902199TruSeq Adapter, Index 10 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC26900.033.98628245
CCGTCTT27400.033.36609347
ATGCCGT27850.033.07471544
CTTGAAA29350.031.85453257
GTCTTCT29300.031.55565649
CTTCTGC29650.031.29951951
GTATGCC29450.031.27778842
TCTTCTG29550.031.17194250
TATGCCG29550.031.17194243
GCCGTCT29300.031.08467746
TGCTTGA30000.031.04935355
TTCTGCT30050.030.9976952
CTGCTTG30350.030.80496254
TCTGCTT30300.030.74193653
CGTCTTC30200.030.50102248
GCTTGAA31000.030.1590556
TCTCGTA28300.029.13543738
CTCGTAT30050.028.81637239
TCGTATG33100.027.72449740
TGAAAAA34750.027.20231259