H32MNAFXY_n01_zt2wtlarvs62.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H32MNAFXY_n01_zt2wtlarvs62.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1408367
Sequences flagged as poor quality	0
Sequence length	75
%GC	52

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG	5977	0.4243922216297315	TruSeq Adapter, Index 10 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4950	0.3514708879148688	No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG	3105	0.22046810241932677	TruSeq Adapter, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG	1603	0.11381976430859285	TruSeq Adapter, Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG	1520	0.10792641406678798	RNA PCR Primer, Index 10 (96% over 28bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TTTCGGA	610	34.509323	1
GTATGCC	2215	31.306562	42
TATGCCG	2220	31.236053	43
ATGCCGT	2260	30.683203	44
CTTGAAA	2355	29.884935	57
GTCTTCT	2320	29.740967	49
GCCGTCT	2395	28.953667	46
CCGTCTT	2425	28.737745	47
TCTTCTG	2410	28.630308	50
TGCCGTC	2435	28.619724	45
CTCGTAT	2335	28.367914	39
CTTCTGC	2470	28.074509	51
TCGTATG	2510	28.03945	40
TCTGCTT	2440	27.995512	53
GCTTGAA	2495	27.931475	56
TGCTTGA	2550	27.599619	55
CGTCTTC	2550	26.923157	48
CGTATGC	2660	26.847372	41
TTCTGCT	2570	26.713636	52
TCTCGTA	2395	26.072706	38