FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1713872
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG65020.3793748891399124TruSeq Adapter, Index 10 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39490.23041393989749526No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG31260.1823940177562852TruSeq Adapter, Index 10 (96% over 28bp)
GTCCTGGAGACAGTGGGGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCT22000.12836431192061018No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCGTAACGACTTCCCCACTGTCTC17250.10064929002866026No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT23750.030.35925547
GTCTTCT23750.030.35925549
ATGCCGT23750.030.35925544
TATGCCG24250.029.87555543
GTATGCC24750.029.5507942
TCTTCTG24800.029.35210450
CTTGAAA24550.029.088957
CTTCTGC25150.028.66927751
TGCCGTC25300.028.499345
TTTCGGA6200.028.3957331
CTCGTAT25050.027.68195539
CGTCTTC26500.027.33895148
GCCGTCT26550.027.15752646
TCGTATG27100.026.98826840
TGCTTGA26750.026.8255155
TCTGCTT26550.026.76770653
TTCTGCT27000.026.704952
GCTTGAA27900.025.96710656
TCTCGTA25300.025.90845538
TCTAGGG2700.025.5571652
Produced by FastQC (version 0.11.2)