FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741603
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG65240.37459742547526614TruSeq Adapter, Index 10 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53060.30466185462473366No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG31650.18172913115101433TruSeq Adapter, Index 10 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG22850.032.31123454
TGCTTGA23250.031.60695355
GTCTTCT23600.031.57676949
TATGCCG23600.031.57586343
GTATGCC23550.031.49640842
ATGCCGT23500.031.26980844
TGCCGTC23700.031.15149745
CTCGTAT22400.031.11120839
TCGTATG24100.030.77761340
GCCGTCT24100.030.6344646
CTTGAAA24150.030.42817957
CCGTCTT24300.030.38232447
GCTTGAA24200.030.3653156
TCTGCTT24400.030.25867553
CGTATGC25300.029.45416641
TTCTGCT25800.028.4830152
TCTCGTA24100.027.9145838
TCTTCTG27250.027.4738350
CTTCTGC27950.026.66231751
CGTCTTC29050.026.0082748
Produced by FastQC (version 0.11.2)