FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1370661
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC64430.4700651729348103No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCA46300.33779322531245876No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT41520.30291954028020057No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC36150.26374136274396076No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAATCAAGAACGAAAGTTAGAGGTTCGAA35640.26002053024051897No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC35560.25943687023997913No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTT34550.25206816273316307No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG33760.2463045202278317No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA33330.2431673477249298No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA32850.2396653877216905No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGATGGTAGTATCTAGGACTACCATGGT31660.2309834452136597No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGAGGTAGTGACGAAAAATAACAATACA30730.22419839770738353No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG26930.19647454768173897No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAG26430.19282667267836467No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTTATATTGGTTGAACTCTAGATA25070.18290445266918662No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAAATAAGACCTCTGTTCTGCTTTCATT24670.17998615266648718No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTAATGATTAATAGAAGCAGTTT24310.17735968266405772No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGGTCCTTGTTAAAGGATTTAAAG24190.17648419266324789No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTA23820.17378476516075092No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAACAAC23090.16845886765582443No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACATCTTTGGCAAATG22760.16605127015359744No Hit
TTCTAGAGCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGAT21840.15933918014738874No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG21350.15576426264408194TruSeq Adapter, Index 10 (96% over 28bp)
GTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACATCTTTGGCAAATGCTTTCGCTTAA20730.15124089763989784No Hit
GTAGTATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAA20500.14956287513834568No Hit
CTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGGTCTTATTTCATTATCCCATGCACAG19340.14109980513051731No Hit
AGCTGGGAGTGGGTAATTTACGCGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA19140.13964065512916762No Hit
GTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAACAACTTTAATATACGCTATTGGAGCT19080.13920291012876268No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGTGGGTAATTTACGCGCCTGCTGCCTTCCTTA18790.1370871426268056No Hit
CTAGATAACATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGATG18700.13643052512619822No Hit
GGCTAAAACCAAGCGATCGCAAGATCGTTATATTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTTGTAC18370.13402292762397122No Hit
GATTTAAAGTGTACTCATTCCAATTACAGGGCCTCGGATATGAGTCCTGTATTGTTATTTTTCGTCACTACCTCC18280.13336631012336383No Hit
GATATGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGTGGGTAATTTACGCGCCTGCTGCC18190.13270969262275648No Hit
CTCCCAGCTCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCATATCCGAGGCCCTGTAATTGGAATGAGT18020.1314694151216092No Hit
CCCATAAGGTTCATGTTTTAATTGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAACTGTTAACGATC17560.12811337011850488No Hit
TCGTTATATTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTTCAAATGTCT17370.12672717761722266No Hit
ATATAACGATCTTGCGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCCCATAAGGTTCATGTTTTAATTGCA17370.12672717761722266No Hit
GATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAAC17080.12461141011526555No Hit
TTCTTGGACCGTCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAATCAAGAACGAAAGTT16830.12278747261357842No Hit
ATACAGGACTCATATCCGAGGCCCTGTAATTGGAATGAGTACACTTTAAATCCTTTAACAAGGACCAATTGGAGG16040.11702383010824705No Hit
GATATAATGAGCCTTTTGCGGTTTCACTTTTAATTCGTGTGTACTTAGACATGCATGGCTTAATCTTTGAGACAA15810.11534580760669487No Hit
CATTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATG15670.11432440260575007No Hit
AGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATCTGCAT15600.11381370010527768No Hit
CTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAACGTTCGTAGTTGAACTTGTGCTTCATACGGGTAGTACAA14940.10899850510082362No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGGTCCTTGTTA14860.10841484510028374No Hit
GCTCTAGAATTACCACAGTTATCCAAGTAACTGTTAACGATCTAAGGAACCATAACTGATATAATGAGCCTTTTG14820.10812301510001378No Hit
ATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAACAACTTTAATATACGC14600.10651795009852912No Hit
GTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCCCATAA14360.10476697009690944No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGTGGGTAATTTACGCGCCTGCTGCCTTCCTTAGATGT14250.10396443759616711No Hit
TCCTTAGATCGTTAACAGTTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAATTAAAACATGAACCT14200.10359965009582968No Hit
TCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTAGATCGTTAACA14050.10250528759481739No Hit
CTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCATGGTAGTCCTAGATACTACCATCAAAAGTTGAT14000.10214050009447996No Hit
ATATTAAAGTTGTTGCGGTTAAAACGTTCGTAGTTGAACTTGTGCTTCATACGGGTAGTACAACTTACAATTGTG13990.10206754259441247No Hit
GATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTT13730.10017064759265785No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGGG400.002595310834.5032463
CCTAGGG450.0046208730.6695542
TTAGGGC703.849076E-529.5742113
ATATAAG10950.028.0437362
CTCGTAT6850.026.69302239
CCGATCT46900.026.2636664
TTCCGAT47100.026.0788882
TCCGATC47450.026.0319543
CTTCCGA47700.025.7602481
CTTGAAA7900.025.32872857
TCTCGTA7150.025.09052338
TATGCCG7850.025.0505743
GTATTTA7350.024.4193691
CGTATGC8100.024.27740541
TATAAGA12750.024.0846183
AAGGGGG6450.024.06939969
GTACATA1151.6912763E-624.0110191
GTATGCC8200.023.9813442
ATGCCGT8350.023.55053744
GCCGTCT8400.023.41035546
Produced by FastQC (version 0.11.2)