FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391277
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG44160.31740623901638565TruSeq Adapter, Index 10 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG33520.24092973577511884TruSeq Adapter, Index 10 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25400.18256608856467835No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG17410.12513683472090747RNA PCR Primer, Index 10 (96% over 28bp)
GTCCTGGAGACAGTGGGGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCT15020.10795837205675074No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT20000.024.66729544
TATGCCG20050.024.6057843
CCGTCTT20300.024.47270247
TGCCGTC20300.024.30275345
CTTCTGC20500.023.89736251
GTCTTCT20550.023.83921849
GTATGCC20650.023.72377442
ATCTTTT47550.023.4351377
GATCTTT51900.023.1992286
CTTGAAA21550.023.05317157
TCTTCTG21300.022.99980750
CTCGTAT20800.022.55750339
TCTTTTT49550.022.3499668
CGTCTTC22150.022.11719748
TTCTGCT21700.022.09889452
GCCGTCT22700.021.8852846
TCGTATG22800.021.48666240
TCTGCTT22350.021.30183653
CTGCTTG24300.021.29611854
CTTTTTT52550.021.0091429
Produced by FastQC (version 0.11.2)