H32MNAFXY_n01_zt2wtlarvs56.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H32MNAFXY_n01_zt2wtlarvs56.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1446910
Sequences flagged as poor quality	0
Sequence length	75
%GC	52

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG	32975	2.278994547000159	RNA PCR Primer, Index 39 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG	17414	1.2035302817728815	RNA PCR Primer, Index 39 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG	11286	0.7800070495054979	RNA PCR Primer, Index 39 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG	8931	0.617246407862272	RNA PCR Primer, Index 39 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG	6530	0.45130657746508074	RNA PCR Primer, Index 39 (96% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5636	0.38951973515975424	No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG	3275	0.22634441672253286	Illumina PCR Primer Index 11 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGG	2774	0.19171890442391026	RNA PCR Primer, Index 39 (96% over 30bp)
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG	2194	0.1516334810043472	RNA PCR Primer, Index 39 (96% over 30bp)
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG	1859	0.12848069333959955	Illumina PCR Primer Index 11 (96% over 29bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CACTATA	180	34.49905	39
GACACTA	315	31.762125	37
CTTGAAA	11400	30.353111	57
ATGCCGT	11490	30.175411	44
GTATGCC	11510	30.152946	42
TATGCCG	11555	30.12509	43
TGCTTGA	11440	29.976608	55
GCTTGAA	11550	29.958916	56
CCGTCTT	11425	29.954535	47
TGCCGTC	11495	29.922184	45
CTTCTGC	11295	29.87272	51
GTCTTCT	11300	29.859503	49
TCGTATG	11620	29.77844	40
CTCGTAT	11355	29.774607	39
TTGAAAA	11720	29.642101	58
TCTCGTA	11010	29.611925	38
TCTTCTG	11430	29.550076	50
CGTCTTC	11515	29.540653	48
GCCGTCT	11575	29.536549	46
CTGCTTG	11495	29.533047	54