FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968067
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20400.2107292160563267No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG18850.19471792758145873RNA PCR Primer, Index 39 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATG1601.8189894E-1228.03192169
GTCTTCT8050.027.00064749
GTATGCC8300.025.3547242
CCGTCTT8600.025.27255647
GCCGTCT8850.024.16882346
CTACATG2300.024.00057469
TATGCCG8900.023.64541243
ATGCCGT9050.023.63470544
TGCCGTC8950.023.51331545
CTCGTAT9250.023.12368239
TGCTTGA9150.023.00055155
TAACCGT750.001993117622.9993636
CTAGACT902.1264165E-422.9993614
CGTCTTC9550.022.75853248
TTTCGGA2800.022.1905651
CTTGAAA9750.021.93898857
CTGCTTG10150.021.75421554
TCTTCTG9700.021.69639450
GATCTTT20250.021.2957066
GATCTGG16600.021.1982086
Produced by FastQC (version 0.11.2)