FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2259835
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78530.3475032469184697No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG53420.23638893990047946RNA PCR Primer, Index 39 (96% over 30bp)
CCCTTGGGAGATCGGAAGAGCACACGTCTCCCTTGGGAGATCGGAAGAGCACACGTCTCCCTTGGGAGATCGGAA33350.14757714611907505Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG24360.10779548064349831RNA PCR Primer, Index 39 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT19500.030.96037547
ATGCCGT19900.030.8581444
GTATGCC19900.030.6847842
TGCCGTC20400.030.1018145
TATGCCG21300.029.15383543
CTTGAAA20750.028.92967457
GTCTTCT20350.028.82019449
GCTTGAA21100.028.44979956
TTTCGGA5350.028.3872131
CTTCTGC20700.028.33289751
ACATATG2450.028.16283269
GCCGTCT21550.028.0151946
TGCTTGA21900.027.88313555
CGTCTTC22400.026.798148
TCTTCTG22100.026.53805450
CTGCTTG23050.026.49254
TTGAAAA23900.025.69416658
CGTATGC25250.024.72976541
TTCTGCT23800.024.64247952
CTCGTAT24150.024.1419539
Produced by FastQC (version 0.11.2)