FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2243169
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA87220.38882491689212895No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG50570.22543999136935292RNA PCR Primer, Index 39 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG26110.11639782825101452RNA PCR Primer, Index 39 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC18850.026.90414442
GCCGTCT19800.025.43904946
TGCCGTC20300.024.98241845
ATGCCGT20500.024.7386944
GTCTTCT21000.024.47878649
GATCTGG42900.024.2863856
GATCTTT54450.024.140176
TGCTTGA21300.024.13401455
ATCTTTT51800.023.8433067
TCGTATG21650.023.74332840
ATCTGTG9500.023.2417897
CGATCTG133450.023.1117125
CTGCTTG22750.022.59580254
GCTTGAA22750.022.59529956
CCGTCTT22400.022.48630147
TCTTCTG22800.022.39493250
ACGTATG3700.022.37857269
CTCGTAT22900.022.14598839
CGATCTT96850.022.0854025
TCTTTTT56350.021.6731768
Produced by FastQC (version 0.11.2)