FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1663746
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50580.30401275194651106No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG19410.1166644427695093RNA PCR Primer, Index 39 (96% over 30bp)
TCCCTGAAGAGATCGGAAGAGCACACGTCTCCCTGAAGAGATCGGAAGAGCACACGTCTCCCTGAAGAGATCGGA18360.11035338326884031Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATAT5500.029.48145968
ACGTATG5450.029.1189169
TTTCGGA4450.027.1467381
AGTCACC12900.026.7438655
AATCGGA8650.024.72802269
CCGTCTT7250.024.2686747
GTCTTCT7700.024.19451149
GTATGCC7450.024.08024442
AAGTCAC15900.023.65065654
CTACATG5250.022.9997269
GCTAGGT750.00199353722.99971841
CGACTAT3750.022.99971867
AAATCGG8600.022.86600168
GCTTGAA8250.022.58154356
TACATGT4750.022.51551468
TCTTTTT37600.022.48045513
TGCCGTC7850.022.4137445
AACTCAC9400.022.38802554
ACTATTC1704.441972E-922.32325769
TATGCCG8100.022.14787943
Produced by FastQC (version 0.11.2)