FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110
Sequences flagged as poor quality0
Sequence length75
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1917.272727272727273No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG21.8181818181818181TruSeq Adapter, Index 11 (96% over 28bp)
AATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTAA10.9090909090909091No Hit
GATAGGGACCAACCTGTCTCGCGACGTTCTGAACCCACCTCAAGGACCACTTTAATTGGCGAAGCCCAACCCGTG10.9090909090909091No Hit
GATCTACACAGGGCTGAACGAACCAGAGGACCAAAAGGAGAGGTTCTTAGAACAGCTTAAGGAGAAGGAGATGGG10.9090909090909091No Hit
CAACAGTAGATCTAATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAACTAAAAAATATAAAAAA10.9090909090909091No Hit
AAAAAAAACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCTAATAAAAAAATAAAAAAAAAAAAA10.9090909090909091No Hit
GAGAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAACCCGTAGCAAGATAAAAAAAATAACACAA10.9090909090909091No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAACAAAAATAAAAAAAAAAAA10.9090909090909091No Hit
GCCTAGATCGGAAGAGCACACGTCTCCCCGACTAGATCGGAAGCTCACACGTCTACACATCTACTTCCCAAGTAG10.9090909090909091Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAGAAAACCACCAGGAGTGAAACAAACAAAAAAAAAAA10.9090909090909091No Hit
AATCTGGGTCGAGCAAAATTCGAAGTGACCTTATAAAATCAAAACAAGTGTAAAATCACAAAAGAAAGGAGAAAA10.9090909090909091No Hit
GATCTGTGCCGGGTGTGCTCCTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAATATAA10.9090909090909091No Hit
AAAAAACACCGGGAGACTTTACATCTACCCATCTAAATACTCATACCATACAACTACCAAAAAAAAAAAAAAAAA10.9090909090909091No Hit
AATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTC10.9090909090909091No Hit
TTTAAAGAAAGCGTAACAGCTCACTGGTCTAAATAAGAGGTTCTGCGGCGAAGATGTAACGGGGCTAAAGTGATG10.9090909090909091No Hit
CCCCACAATGAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAATACAAATAAAAACTACAGTCACA10.9090909090909091No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAA10.9090909090909091No Hit
GTGCTCTTCCGATCTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGGGAAAAAAACCCCTTTTGGTGTAAAATAA10.9090909090909091No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAATAAAAAATA10.9090909090909091No Hit
AAACTAAAATCCAAATAGAAAGACGATAATATAGAATACAGTATAAACCTTCAAAAAAAAAAATCTTATAGACAT10.9090909090909091No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAA10.9090909090909091No Hit
ACCCGGGGACCTTCGGTGCCCGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTG10.9090909090909091No Hit
CACACCGGCTGAGATCGGAAGAGCCCACGAGACAAGAGGCAATATCGTATGCCGTCTTCTTCTTGAAAAAAAAAA10.9090909090909091Illumina Paired End PCR Primer 2 (95% over 21bp)
GTGCTCTTCCGATCTCCCTTGGGAGATCGGAAGAGCAGACGTCCTGTCTCTTATACACATCTCCGAGCCCACGAG10.9090909090909091No Hit
CTTCAGATCTAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGAGCCGAAGAAACATAAAAAA10.9090909090909091No Hit
CGTCTCCCGTGGTAGATCGGAAGAGCACACGTCTCCCGTGGTAGCTCGCACTTGAACACATCTCCGAGAGAAGCA10.9090909090909091Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCACTCGGACCCCACGCAGCGTTCATTCTAGCCTCCTACGGCGCGGCCGCGGCCGTACTCGCCGGTCTCATCGGT10.9090909090909091No Hit
GTTTCAGGATCTATTTCACTCCCCTCCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCG10.9090909090909091No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAATAAAAAAAAAAAAA10.9090909090909091No Hit
CTTACGATCTATAAATTTTATAATAAAAAAAAATAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.9090909090909091No Hit
CATAAGATAACACTATTGATATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAATACAAACCTACTAAC10.9090909090909091No Hit
CCTCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAGACGCAACAACGCCGAGCGAAAATCAAAACTAAACCGACC10.9090909090909091No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAATAAAAA10.9090909090909091No Hit
GCTTGCAGCATGGCGGCGCGCTGTTCCCGGTCGATGGCCGCGCGCGGCATGCTCGCGTCGCGCTCCGACGCCTGT10.9090909090909091No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGAAAAACCCCCCCAAACCAATAATATTTTAAAAA10.9090909090909091No Hit
GCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA10.9090909090909091No Hit
TATTCCCCCCGGTTCGCGGTTTCAGGAGGAGACTACAGCCCAGCTACCGAGTCTTCCTGTCTCTTATACACATCT10.9090909090909091No Hit
GTTAAGACTACGACGGTATCTGATCGTCTTCGATCCCCTAACTTTCGTTCCTGATCAGCGAAAACGTCCTTGGCA10.9090909090909091No Hit
AAATAGAACAAAACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAACAAAAAACAAAAAAA10.9090909090909091No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAGGGGAAAGACCCCCGGGGGGTGGGTTTTAAAAA10.9090909090909091No Hit
ATATTTGAGTCTAGTTCGTTATCGGAATTAACCAGACAAATCACTCCACGAACTAAGAACGGCCATGCACCAACA10.9090909090909091No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAA10.9090909090909091No Hit
ACCCAAGAAAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAACAAAAGAATAAAAAAAAAAAAATAA10.9090909090909091No Hit
ATAAACAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCAACAGCAAAAAAAAAAAAAAAAAAACG10.9090909090909091No Hit
CGGCTGGGAGACGTGTGCTCTTCAGATCTCTTCTGGGATACGAGGCCTCTTGACATCTCTACAGGTCGAATAAAA10.9090909090909091Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTTGAGATCGGAAGAGCACACGTCTCCCGGTTGAGATCGGAAGAGCTGTCTCTTATACACATCTCCGAGCCCACG10.9090909090909091Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCGTCGAATTGGTCCTTGTTAAAGGATTTAA10.9090909090909091No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAACAAAAAAAAAAAAAAAAAAAAAAAAAA10.9090909090909091No Hit
ATTATAAGGACACTAGCTTCTTAAATGGACAAATTGAGTCTAGCAATAATGAGATTGAGCAATAACAGGTCTGTT10.9090909090909091No Hit
GCAAAGGCCTCCTCTACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAACCCACAGGCAGGAATAAATA10.9090909090909091No Hit
GTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAATTAAAAC10.9090909090909091No Hit
GGGATAAGTACACTAGCATCATAAAAGCACAAATAGACTCACGCAATAAAGAGAAAGAGCAATAACAGTACTGTC10.9090909090909091No Hit
CACCGGGGAGAAATAGGCGAAACAGCTCAATACTGGGAGAGGCGGTAAGTACCGACTGCGCCTGGTCGACACTAA10.9090909090909091No Hit
AAAAAAATCAAACCAATTATCAACCTAAAAAAAAAAAAAAAAAAAAAACAACCAAACAAAACAAAAAAACAAAAA10.9090909090909091No Hit
CAAAACATCAACAAGAGTTGCACATAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAACACAAACAAGAA10.9090909090909091No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA10.9090909090909091No Hit
AACATAACAAATAGCACAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAACTAAAAAAAATATAAAAAAAA10.9090909090909091No Hit
GTCATCTTAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATCGATATTTTATAAAAACTAAACT10.9090909090909091No Hit
AACATGCCTCGACCTCCCACATCCATTGCATACACAGGCGTAACCCCTGCCAAGCGTGGCCGCACCCCAGAGGCT10.9090909090909091No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTACGCATACCAATGTAAATTCATACACACTAAAATATCAAT10.9090909090909091No Hit
GTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTATGGTTCCTTAGATCGTTAAC10.9090909090909091No Hit
GAACAGAGGTCTTATTTCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTT10.9090909090909091No Hit
AAAAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.9090909090909091No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAGAAA10.9090909090909091No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAGAAAAACACAAGAAAGTAAAAAAAAAAAGAAAAA10.9090909090909091No Hit
GCCTAAGGCTTTTCGCCGAGGACCTGGAAACCCTCCTGGTCCGCTTCCTCAACGAGCTGATCTACCTGATCCAGA10.9090909090909091No Hit
GAAAAAAAATATTAAAAGATAAAGAGAAAAATATTAATATAAACATTTTTTGGACAATATTAATACTACTCAATT10.9090909090909091No Hit
GTCGCGCGATCCCACACCACCGTCGTCTCCCGCTGGATCGTGATGCCGATGGCCGCCACCTGGCGCGGATCGACG10.9090909090909091No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAGCATAAAGGTAAAGTTCAACGATAAATAAGCA10.9090909090909091No Hit
CTTCCGATCTTCCCAGGGTCTCATTCGATTATTTGCTACTACCACCAAGATCTGTACCAATGGCAGCTCCATGCA10.9090909090909091No Hit
ATAAAAGACATAGCTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATACAAACAAAATATAAAAAAA10.9090909090909091No Hit
ACACAACTAAGACCACACTTCTCTTAAGAAAAAAAAAAAAAAAAAAAAAAAAAAACACATATAAAATAAAAAAAA10.9090909090909091No Hit
ATTAAAACATGAACCTTATGAGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTTATATTGGT10.9090909090909091No Hit
ATACACATCTACGAGCCCACGAGACCGAGGCAGATGTCGTATGCCTACTTCAACTTAAAAAAAAAAAAGTGGTCT10.9090909090909091No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTATGTAGAGCACCCCCCCGCAGGTGGTTATATAAAAAAATAAAGAGA10.9090909090909091No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAA10.9090909090909091No Hit
ATAAAATGTTCATCAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCACAACAATTAAA10.9090909090909091No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTATGGGGG10.9090909090909091No Hit
GATCAAGAGGTAATGATTAACAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAAATTCTTGGACC10.9090909090909091No Hit
TAATGCGACTGTAACGCTCTCTGGTAGGCCTTCCCAGAACGTTAACCTAAGCGAGTCTTTTGTAACTCCACGTGA10.9090909090909091No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAA10.9090909090909091No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAACCACCCCACCCAAAAAAAAAAAAAAAAAAAAAAA10.9090909090909091No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG10.9090909090909091TruSeq Adapter, Index 10 (96% over 28bp)
TTTGTCTGCTTAATTGCGATAACGAACGAGACCTTAACCTGCTAAATAGCCAGGCCCGCTTTGGCGGGTCGCCTG10.9090909090909091No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAA10.9090909090909091No Hit
TTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTCAAAACCCAAAGGCTATTTACCAAAAAAACA10.9090909090909091No Hit
GATCGGAAGAGCACACGTCTCCCAGGGCCGATCGCAATATCACAACTCACCCCCGACAACTCCGAAAACCAAAAG10.9090909090909091No Hit
CCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAGCCCAAAAGCTTTGGTTTCCCGGGAAGCGACTGAGAG10.9090909090909091No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGAAAAAGACAGAAGATAATATAAAAATATCTA10.9090909090909091No Hit
GTCTCACGACGTTCTGAACCCAGCTCACGTACCACTTTAATCGGCGAACAGCCGAACCCTTGGGACCTTCTCCAG10.9090909090909091No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers