FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1581929
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG81940.5179752062197481TruSeq Adapter, Index 1 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39220.24792515972587897No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG38160.24122447973328764TruSeq Adapter, Index 1 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG23600.14918495077844834TruSeq Adapter, Index 1 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG21020.13287574853232983RNA PCR Primer, Index 1 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTAGT501.8581393E-434.511351
GTATGCC29550.031.28875442
TCGTATG29700.030.8984140
TGCTTGA29550.030.82273155
CGTATGC30300.030.74199541
CTTGAAA29850.030.62756757
CCGTCTT30200.030.38684847
GTCTTCT29700.030.20240849
CTCGTAT29000.030.09771339
ATGCCGT30900.029.8101244
TGCCGTC30800.029.79489545
TCTCGTA29450.029.28637738
TATGCCG31750.029.22937243
ACGTATG3600.028.75127869
CTGCTTG31950.028.6153954
TCTGCTT31950.028.29144353
GCCGTCT32450.028.17358646
TTCTGCT32600.027.62151752
GCTTGAA33350.027.51672656
TTTCGGA6900.027.5090481
Produced by FastQC (version 0.11.2)