FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1796225
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG23690.13188770894514884TruSeq Adapter, Index 1 (96% over 28bp)
ATTGAAGACTAACTACTGCGAAAGCATTTGCCAAGGATGTTTTCATTGATAAGGAACGAAAGTTAGGGGATCGAA18180.10121226461050259No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGATA2500.033.12101469
AGTTTCG3450.028.99927322
TGCGCTA6850.027.7015810
CTCTACT6900.027.0166231
ATCTGTG4350.026.9648447
GTATGCC9600.026.95245242
TGCCGTC9700.026.6745945
GCGCTAT7000.026.61510311
CGATCTG55550.026.146065
TGCTTGA9900.026.1357155
GCCGTCT9800.026.0503746
ACTTGTG7100.025.7528765
ATGCCGT10100.025.61817244
CCGATCT142750.025.5479164
GGTATAA3150.025.18984830
GCTATCG7300.025.04871413
ATCTTTT11600.024.98352412
GCTTGAA10500.024.97080256
TAGTGCG1251.2649798E-724.8407610
GATCTGG21550.024.6536756
Produced by FastQC (version 0.11.2)