H32MNAFXY_n01_zt2wtlarvs40.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H32MNAFXY_n01_zt2wtlarvs40.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	660305
Sequences flagged as poor quality	0
Sequence length	75
%GC	53

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG	8147	1.2338237632609173	RNA PCR Primer, Index 27 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG	3893	0.5895760292592059	RNA PCR Primer, Index 27 (96% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2858	0.43283028297529175	No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG	2548	0.3858822816728633	RNA PCR Primer, Index 27 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG	1833	0.27759898834629454	RNA PCR Primer, Index 27 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG	1341	0.20308796692437586	RNA PCR Primer, Index 27 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG	743	0.11252375796033652	RNA PCR Primer, Index 27 (100% over 28bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGTA	40	5.000089E-5	43.124435	38
AGTCGTA	80	1.6389095E-9	38.81199	67
GTCTTCT	2590	0.0	35.831573	49
CCGTCTT	2635	0.0	35.612434	47
ATGCCGT	2675	0.0	35.466824	44
GCTTGAA	2620	0.0	35.42129	56
TGCCGTC	2675	0.0	35.079914	45
CTTGAAA	2655	0.0	34.95434	57
GTATGCC	2725	0.0	34.68945	42
TTCTGCT	2640	0.0	34.630222	52
ACTTGAT	60	1.3418059E-5	34.499546	40
CGCGTAT	60	1.3418059E-5	34.499546	39
GCGTATG	60	1.3418059E-5	34.499546	40
TATGCCG	2745	0.0	34.436707	43
GCCGTCT	2715	0.0	34.43601	46
TATCTCG	2610	0.0	34.367367	36
TGCTTGA	2720	0.0	34.119034	55
CTTCTGC	2710	0.0	34.11763	51
TCTTCTG	2735	0.0	34.058052	50
TTGAAAA	2765	0.0	33.938072	58