FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2051307
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA148100.721978718933831No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31450.15331688528338272No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23600.11504860072139372No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA8600.031.7069381
TGCCGTC9400.023.85609645
ATGCCGT9750.023.70740544
CCGTCTT9450.023.36479847
AATCTAA8100.022.99972369
GTATGCC10100.022.88586442
AGTCACC10650.022.67578355
GCGAATA8050.022.28544669
ATCTTTT77450.022.00543812
TCTTTTT79350.021.6959213
ACGTATG6850.021.65667369
CTTGAAA10050.021.62660857
GATCTTT86600.020.7555246
GTCTTCT9150.020.73745749
CTTTTTT82050.020.68764114
AGACAAT5700.020.57870167
TATGCCG11650.020.13709843
GCCGTCT11000.020.07248546
ATATACC5850.020.0510447
ACATATG3100.020.03201769
Produced by FastQC (version 0.11.2)