FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949581
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGGT953.2016942E-729.0537833
CGATCTG38150.028.666735
CGATCTT16900.028.375375
ATCTGAG2950.028.0674347
CTATAGA500.00772083927.6098181
ATCTGTG3400.027.3967027
TTCCGAT98000.027.037812
CCGATCT97850.027.0087384
CACTGTG3450.026.999657
ACTGTGC3500.026.613948
TCCGATC100150.026.3884663
CTTCCGA102800.026.0520671
CTGTGCA4200.025.4652969
ATCTTGG2001.8189894E-1224.1496857
GATCTTG5650.023.813856
GATCTGA10750.023.748536
GATCTTT7000.023.6568346
ATCTAGC4200.022.99977
TGCCGTC3950.022.70856745
GTCTAGG2450.022.538631
Produced by FastQC (version 0.11.2)