FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658043
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19880.3021079169598339No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG14700.22338965690691945RNA PCR Primer, Index 27 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTCT4900.029.5724749
AGTTGTC500.00773122527.60097151
GCACAGT500.00773409327.5988756
TGCTTGA5450.026.58809355
GTATGCC5750.026.39892442
CCGTCTT5700.026.02525547
TTCTGCT5650.025.64692152
TGCCGTC5950.024.93175745
TAGTTAC2100.024.64559715
ATTTACG700.001333557424.64185316
GCCGTCT6050.024.51966346
ATCTTTT14600.024.3417512
TATGCCG6100.024.31868243
ATAGTTA2150.024.07244514
TATCTCG5800.023.79213336
TCTGCTT5950.023.77394953
ATGCCGT6400.023.71778344
CTTCTGC6250.023.18481651
CGTTATA1803.4197E-1023.0025610
AACTGAC902.1245044E-423.00080965
Produced by FastQC (version 0.11.2)