FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1807515
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78580.43474051390998136No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG44180.24442397435152685RNA PCR Primer, Index 27 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC14050.028.48365442
TTTCGGA6000.028.1894871
GTCTTCT14200.027.21094949
CCGTCTT15450.025.9026147
TCCTATA3100.025.59647669
ATGCCGT15550.025.51417544
TATGCCG15850.025.46658143
TCCTATG3450.024.99970869
CTTGAAA16250.024.8397157
TGCCGTC16050.024.7193445
AATCGGA8400.023.82115269
TTTATAC1452.0161679E-823.7934843
GCCGTCT17150.023.33500746
TATCTCG17150.023.13384236
TCTTCTG16800.022.99973350
ATCTTTT52350.022.73675312
TGCTTGA18050.022.55375355
CTTCTGC17700.022.0251751
CTGCTTG18100.021.91963654
GCTTGAA18450.021.87779456
Produced by FastQC (version 0.11.2)