FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2153423
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG49650.23056315456833143RNA PCR Primer, Index 27 (96% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42950.1994498990676704No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG22220.1031845577947296RNA PCR Primer, Index 27 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC19850.030.06753545
CCGTCTT19650.030.02242547
GCCGTCT19800.029.96922346
GTCTTCT19550.029.64659149
TATCTCG18950.029.49293536
ATGCCGT20250.029.47360844
GTATGCC20550.029.04333742
TATGCCG21200.028.47832343
CTTCTGC21150.027.89317751
GCTTGAA22050.026.91113956
CTTGAAA22550.026.1614557
TCCTATA6550.025.80935169
TCGTATG22900.025.761640
CGTCTTC22500.025.60626248
TCTGCTT22850.025.51600653
TCTCGTA22050.025.34653738
TCCTATG3950.025.32930269
TCTTCTG23100.025.23985950
CTCGTAT23000.024.89960539
TGCTTGA24200.024.80539355
Produced by FastQC (version 0.11.2)