FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887414
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC27980.3152981584694404No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT18760.21140076672218377No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA18350.20678060071173096No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCA18160.20463954817030158No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC17680.1992305733287958No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA16390.18469395344224906No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGATGGTAGTATCTAGGACTACCATGGT15640.1762424302523963No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC15500.17466481259029043No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG15210.17139689029021404No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTT15010.16914315077291997No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAATCAAGAACGAAAGTTAGAGGTTCGAA14250.1605789406072025No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG13750.15494459181396733No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTA12260.1381542324101265No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAG11550.13015345712373255No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAACAAC11500.12959002224440902No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTTATATTGGTTGAACTCTAGATA11220.12643478692019733No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTAATGATTAATAGAAGCAGTTT11150.1256459780891444No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGAGGTAGTGACGAAAAATAACAATACA11090.12496985623395619No Hit
GTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACATCTTTGGCAAATGCTTTCGCTTAA10600.1194481944165857No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGGTCCTTGTTAAAGGATTTAAAG10400.11719445489929164No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAAATAAGACCTCTGTTCTGCTTTCATT10370.11685639397169753No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACATCTTTGGCAAATG9840.11088398425086825No Hit
GTAGTATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAA9410.10603844428868599No Hit
TTCTAGAGCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGAT9280.10457351360244485No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA1951.8189894E-1224.778661
GAGACTA700.00133371524.64251537
ATGTACG851.4392156E-424.3622111
CGGTCCA6950.023.8283510
GGTCCAA6850.023.67253911
AACGGGT11250.022.99968122
GGTAACG11650.022.50612426
GTTCGAA6750.022.48857769
AAAGTTA6600.022.47696159
GGGTAAC11550.022.40228525
GGAATCA11450.021.99532934
TCCTATA1103.2661792E-521.9542469
GATGTTT7250.021.8893536
TATGCCG3800.021.7891743
ATATAAG4450.021.7087882
TGAGAAA10700.021.60250365
ACTTAGG800.00289252221.570711
TAACGGG12000.021.562228
AGAAACG10100.021.51950567
GAGAAAC10750.021.50202866
Produced by FastQC (version 0.11.2)