FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2046897
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG110750.541062886896605RNA PCR Primer, Index 26 (100% over 29bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA99990.4884955129642576No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG61450.3002105137679131RNA PCR Primer, Index 26 (100% over 29bp)
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG55460.2709467061605933RNA PCR Primer, Index 26 (100% over 29bp)
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG31390.15335407692717318RNA PCR Primer, Index 26 (100% over 29bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG27900.13630387850487835RNA PCR Primer, Index 26 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC70000.032.28162842
TATGCCG69750.032.19948643
ATGCCGT68700.031.78770344
CCGTCTT59100.031.75583647
TGCCGTC63000.031.48759545
GCCGTCT59450.031.3367646
CTTGAAA68350.031.04193357
TGCTTGA64800.030.87913955
TTTCGGA9400.030.845121
TCGTATG62600.030.80701840
TGAAAAA95150.030.49294759
GCTTGAA69650.030.31394656
GTCTTCT52650.029.74881449
CGTATGC73350.029.63143241
CTTCTGC54150.029.43443351
TCTCGTA62400.029.35770638
TTGAAAA78150.029.09166758
CTCGTAT65000.028.9795439
CATCTCG66750.028.83999336
GCATCTC68300.028.53907835
Produced by FastQC (version 0.11.2)