FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1181331
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32670.27655246497383035No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC13890.11757923901091227No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATG1700.034.49923769
TAGTATG450.004623241330.6659940
ATGCCGT8100.028.53640644
TAGGATA1457.039489E-1026.1729434
GTCTTCT6950.024.81959749
GTATGCC9350.024.72137842
ACTAGAC700.001334040324.64231356
CGTTTAT851.4435493E-424.35240437
TATGCCG9800.024.2902843
TGCCGTC8700.024.18912145
CCGTCTT8050.023.57090847
ATCTTTT28900.023.04123512
TTACACG902.1261108E-423.0004654
GAGCATA2450.022.53011569
TGCTTGA9100.022.36763855
GATCTTT30600.022.32492611
AGACGTA2350.022.0207967
ATTAAGC1103.2664997E-521.954993
TCTTTTT30250.021.89889313
GATCTGG18300.021.868376
Produced by FastQC (version 0.11.2)