FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66877
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC3000.44858471522347No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA1690.2527027229092214No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTT1640.24522631098883024No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCA1630.24373102860475201No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAATCAAGAACGAAAGTTAGAGGTTCGAA1600.23924518145251727No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGATGGTAGTATCTAGGACTACCATGGT1590.23774989906843907No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT1580.23625461668436085No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC1550.2317687695321261No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC1540.23027348714804793No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA1400.20933953377095266No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG1360.2033584042346397No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAG1340.20036783946648326No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG1330.198872557082405No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1270.1899008627779356No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCACTCTAATTTGTTCAAAGTAATAGTA1240.18541501562570092No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTAATGATTAATAGAAGCAGTTT1180.17644332132123153No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGGTCCTTGTTAAAGGATTTAAAG1110.16597634463268388No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAAATAAGACCTCTGTTCTGCTTTCATT1070.15999521509637096No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGTTATATTGGTTGAACTCTAGATA1060.1584999327122927No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGAGGTAGTGACGAAAAATAACAATACA1050.15700465032821448No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACATCTTTGGCAAATG990.14803295602374508No Hit
CTAGATAACATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGATG990.14803295602374508No Hit
GTAGTATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAA980.14653767363966685No Hit
GTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACATCTTTGGCAAATGCTTTCGCTTAA960.1435471088715104No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAACAAC940.14055654410335391No Hit
AGCTGGGAGTGGGTAATTTACGCGCCTGCTGCCTTCCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA900.13457541456704097No Hit
CTCCCAGCTCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCATATCCGAGGCCCTGTAATTGGAATGAGT880.13158484979888452No Hit
GTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAACAACTTTAATATACGCTATTGGAGCT870.1300895674148063No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGTGGGTAATTTACGCGCCTGCTGCCTTCCTTA870.1300895674148063No Hit
ATACAGGACTCATATCCGAGGCCCTGTAATTGGAATGAGTACACTTTAAATCCTTTAACAAGGACCAATTGGAGG870.1300895674148063No Hit
TTCTAGAGCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGAT840.12560372026257158No Hit
TTCTTGGACCGTCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAATCAAGAACGAAAGTT820.12261315549441512No Hit
GATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAAC790.11812730834218042No Hit
TCGTTATATTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTTCAAATGTCT790.11812730834218042No Hit
GATTTAAAGTGTACTCATTCCAATTACAGGGCCTCGGATATGAGTCCTGTATTGTTATTTTTCGTCACTACCTCC780.11663202595810218No Hit
CTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGGTCTTATTTCATTATCCCATGCACAG780.11663202595810218No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGGTCCTTGTTA760.11364146118994572No Hit
GATATGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGTGGGTAATTTACGCGCCTGCTGCC750.1121461788058675No Hit
GTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTC720.1076603316536328No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTAC720.1076603316536328No Hit
AGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATCTGCAT720.1076603316536328No Hit
ATATAACGATCTTGCGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCCCATAAGGTTCATGTTTTAATTGCA710.10616504926955456No Hit
CCCATAAGGTTCATGTTTTAATTGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAACTGTTAACGATC700.10466976688547633No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGTGGGTAATTTACGCGCCTGCTGCCTTCCTTAGATGT690.10317448450139809No Hit
GGCTAAAACCAAGCGATCGCAAGATCGTTATATTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTTGTAC690.10317448450139809No Hit
GCTCTAGAATTACCACAGTTATCCAAGTAACTGTTAACGATCTAAGGAACCATAACTGATATAATGAGCCTTTTG670.10018391973324163No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG208.4557745E-569.05
TGTGGTC200.00732891751.758
AATGGCT200.00732891751.7510
AGACCGA200.00732891751.756
AGGGATT306.2686816E-446.00000415
TGCCCTA801.5916157E-938.812533
ACCGGTA557.2905805E-637.6363615
CCGGTAA557.2905805E-637.6363616
TATCAAC852.8812792E-936.5294138
CCTATCA852.8812792E-936.5294136
AGAAACG1151.8189894E-1236.067
ATAAGAA601.3237792E-534.5000044
AACTCCA601.3237792E-534.5000049
CTTAACC400.002578954734.53
AGATCTT905.0367817E-934.517
TGTCTGC1003.6743586E-1034.529
GCCCTAT905.0367817E-934.534
GAATCAG1550.033.38709635
AACGGGT1550.033.38709622
CGGGGAA1550.033.38709631
Produced by FastQC (version 0.11.2)