FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718374
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA115821.6122521138014463No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACAAT1359.094947E-1230.66694567
GAGTTAG1103.646528E-828.2373561
TGCAATA756.1599625E-527.6002569
AACTATC500.007735302727.598337
CGCGTAT658.6569827E-426.53685439
TTTCGGA2300.025.5091111
ACGTATG1401.385888E-824.64308269
GTTAGCC2100.024.643083
TGTCATT902.1251453E-423.00020830
ACAGATT1052.280306E-522.9986088
TCTTTTT14300.022.44195413
ATCTTTT14600.022.2171712
TTAGCCG2350.022.0214774
CGTCTTA2251.0913936E-1121.46536648
TTACGAC2750.021.3274654
ATTAGAC1154.599224E-521.000193
CTTTTTT15700.020.88034614
CTAGTCG850.004118485420.29430226
CCATACG1206.372122E-520.1293859
AGACGTA2402.7284841E-1120.12518367
Produced by FastQC (version 0.11.2)