FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4727778
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66040.13968506981503784No Hit
GTATAGATTCTGTTTGACGACCCGCTGAATTTAAGCATATTACTAAGCGGAGGAAAAGAAATCAACTGAGATTCC62280.1317320737141211No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATTC20750.028.9308724
ACGACCC23750.025.56562417
CGACCCG24200.024.94767418
CTGTTTG25300.024.82013910
GACGACC25400.024.17651416
CTATACT15000.024.1506254
ACCCGCT25850.023.75564420
GCCGTCT17800.023.64543246
GACCCGC26150.023.61504219
GATCTGG64200.023.5904856
TGCCGTC19400.023.29576345
ACCTAGG4000.023.295741
CCGCTGA27050.022.8293322
CGCTGAA27050.022.8293323
TAAGATG19550.022.7640915
ATGCTAG12600.022.72605735
ATCTGGC22500.022.6927227
TATGCCG22350.022.69066243
CAATACT23900.022.375064
GTATTGA19750.022.0179141
Produced by FastQC (version 0.11.2)