FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1429175
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160431.1225357286546433No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28440.19899592422201617No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19800.1385414662305176No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18370.1285356936694247No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA5350.030.9657061
CTAGGTT500.00773358727.60142515
GTATGCC13850.024.16201442
ATGCCGT12650.023.99956344
CCGTCTT11850.023.87298247
ATCTTTT63050.023.200217
TGCCGTC12650.023.18139645
GATCTTT65900.023.1391816
CGCGTAT2550.022.9995839
TCTTTTT64800.022.62698
TCGTATG12300.022.1581340
TTATCGT2500.022.08114212
TATGCCG14850.022.07030543
GTATGGC1906.7484507E-1021.78907639
CTTGAAA15150.021.4055557
GTCTTCT11500.021.2996149
CTTTTTT68400.021.2861889
TGCTTGA14050.021.1170555
CATCTCG13450.021.03307336
GCCGTCT13100.020.80496446
Produced by FastQC (version 0.11.2)