H32MNAFXY_n01_zt2wtlarvs25.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H32MNAFXY_n01_zt2wtlarvs25.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1069564
Sequences flagged as poor quality	0
Sequence length	75
%GC	49

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG	2061	0.19269534127925023	RNA PCR Primer, Index 26 (100% over 29bp)
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAATCAAGAACGAAAGTTAGAGGTTCGAA	1924	0.17988638361051795	No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTT	1737	0.16240262387290522	No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA	1297	0.12126436566675766	No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG	1271	0.11883346859093986	No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG	1115	0.104248086136033	No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG	1099	0.10275214947399126	RNA PCR Primer, Index 26 (100% over 29bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTATAT	50	1.8548347E-4	34.52054	1
TGCCGTC	1190	0.0	33.91973	45
CCGTCTT	1145	0.0	33.444984	47
ATGCCGT	1295	0.0	33.03432	44
GTATGCC	1325	0.0	32.546753	42
CTTCTGC	1080	0.0	31.944033	51
CGTCTTC	1100	0.0	31.676867	48
TATGCCG	1345	0.0	31.54978	43
TCTTCTG	1120	0.0	31.419237	50
GCCGTCT	1200	0.0	31.337097	46
GTCTTCT	1165	0.0	30.501755	49
TGCTTGA	1390	0.0	29.535591	55
CTTGAAA	1435	0.0	29.330633	57
CTCGTAT	1295	0.0	28.77183	39
CTGCTTG	1260	0.0	28.74963	54
CGTATGC	1480	0.0	28.438824	41
TTTCGGA	235	0.0	27.910221	1
GCTTGAA	1490	0.0	27.784876	56
TGAAAAA	2030	0.0	27.531664	59
TCTCGTA	1345	0.0	27.445744	38