FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences652799
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44750.6855096285380339No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG34380.5266552185282146RNA PCR Primer, Index 17 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG16910.25903838700733306RNA PCR Primer, Index 17 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG12870.19715103730244687RNA PCR Primer, Index 17 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG8340.12775754864820565RNA PCR Primer, Index 17 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG6900.10569869132765214RNA PCR Primer, Index 17 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATAT400.002595629834.49969540
TACAATA400.002595629834.49969551
GCGTATC450.00462144430.66639364
CCGTCTT13650.028.30744247
GTCTTCT13750.027.59975649
GTATGCC14250.027.59975242
GCCGTCT14050.027.25598746
TCTTCTG13950.027.2040650
TCGTATG14100.027.15933440
TGCTTGA14650.027.0816755
GTATTGG906.880098E-626.8433761
CTCGTAT14100.026.66997739
TATGCCG14750.026.66417143
GTACAAA1057.649978E-726.2955511
AATCTCG13800.026.2497736
ATGCCGT15200.026.10174244
TTCTGCT14500.025.93425452
AGTGTGC1359.411451E-925.55532841
CGTATGC15400.025.53873341
TTGAAAA16350.025.10986958
Produced by FastQC (version 0.11.2)