Basic Statistics
Measure | Value |
---|---|
Filename | H32MNAFXY_n01_zt2wtlarvs23.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 636310 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3128 | 0.4915842906759284 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG | 2584 | 0.40609137055837563 | RNA PCR Primer, Index 17 (96% over 28bp) |
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG | 1204 | 0.1892159482013484 | RNA PCR Primer, Index 17 (96% over 28bp) |
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG | 967 | 0.15196995175307632 | RNA PCR Primer, Index 17 (96% over 28bp) |
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC | 775 | 0.12179597994688124 | No Hit |
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT | 677 | 0.10639468183746917 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTATATA | 35 | 0.0013476593 | 39.42682 | 41 |
CACTATA | 35 | 0.0013476593 | 39.42682 | 39 |
GTATGCC | 1135 | 0.0 | 34.346493 | 42 |
GCTTGAA | 1170 | 0.0 | 33.908752 | 56 |
GTCTTCT | 1090 | 0.0 | 33.54897 | 49 |
TGCTTGA | 1175 | 0.0 | 33.47085 | 55 |
TATGCCG | 1175 | 0.0 | 33.47085 | 43 |
CCGTCTT | 1135 | 0.0 | 33.43464 | 47 |
ATGCCGT | 1190 | 0.0 | 33.048954 | 44 |
AGGATAT | 220 | 0.0 | 32.932945 | 34 |
CTTCTGC | 1135 | 0.0 | 32.522785 | 51 |
GGATATA | 170 | 0.0 | 32.4717 | 35 |
TGCCGTC | 1175 | 0.0 | 32.296436 | 45 |
GCCGTCT | 1200 | 0.0 | 31.623596 | 46 |
CGTCTTC | 1180 | 0.0 | 31.282509 | 48 |
CTTGAAA | 1315 | 0.0 | 30.694454 | 57 |
CCTATAT | 45 | 0.00462216 | 30.665304 | 2 |
TCTCGTA | 1130 | 0.0 | 30.532017 | 38 |
TCTTCTG | 1215 | 0.0 | 30.381369 | 50 |
CTCGTAT | 1125 | 0.0 | 30.35865 | 39 |