FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636310
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31280.4915842906759284No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG25840.40609137055837563RNA PCR Primer, Index 17 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG12040.1892159482013484RNA PCR Primer, Index 17 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG9670.15196995175307632RNA PCR Primer, Index 17 (96% over 28bp)
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC7750.12179597994688124No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT6770.10639468183746917No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATATA350.001347659339.4268241
CACTATA350.001347659339.4268239
GTATGCC11350.034.34649342
GCTTGAA11700.033.90875256
GTCTTCT10900.033.5489749
TGCTTGA11750.033.4708555
TATGCCG11750.033.4708543
CCGTCTT11350.033.4346447
ATGCCGT11900.033.04895444
AGGATAT2200.032.93294534
CTTCTGC11350.032.52278551
GGATATA1700.032.471735
TGCCGTC11750.032.29643645
GCCGTCT12000.031.62359646
CGTCTTC11800.031.28250948
CTTGAAA13150.030.69445457
CCTATAT450.0046221630.6653042
TCTCGTA11300.030.53201738
TCTTCTG12150.030.38136950
CTCGTAT11250.030.3586539