FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822345
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41470.5042895621667305No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG21600.2626634806559291RNA PCR Primer, Index 17 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG10520.12792684335649881RNA PCR Primer, Index 17 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG8940.10871349616037064RNA PCR Primer, Index 17 (96% over 28bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8370.10178209875417253No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA4150.030.7700181
CTAGGTC450.004622113430.66642221
TCCTATA605.419198E-428.751522
CAACTTA500.007733957427.59978314
ATCTTTT31200.026.20652212
ATGCCGT9300.025.59657344
TATGCCG9950.025.31135743
GTCTTAC1501.036824E-925.3090321
GTATGCC9700.025.25237742
GATCTTT33700.024.9790311
TCTTTTT32650.024.72568313
TATATAC700.001333113424.644163
TATTCGT1401.3867975E-824.642667
CCGTCTT9550.024.5652547
AGAAATG4800.024.43730769
TGCCGTC9750.024.4151945
GTCTTCT9600.024.07793449
TCGTAAT1151.6967842E-623.99981135
TACTAAT1151.6967842E-623.99981141
TCGTATG10250.023.22420540
Produced by FastQC (version 0.11.2)