FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2573777
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA131880.5123987043166521No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG66040.25658788620770173RNA PCR Primer, Index 17 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG30160.11718186929170632RNA PCR Primer, Index 17 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT26000.031.58038747
ATGCCGT28800.029.22881344
GTCTTCT26900.028.9847849
TATGCCG28900.028.88892443
TTTCGGA8200.028.6199721
GTATGCC29450.028.58369642
CTTGAAA31250.027.82046357
TGCTTGA30400.027.8039455
GCCGTCT29650.027.6927546
TGCCGTC29950.027.53055445
GCTTGAA31300.027.2249156
CTTCTGC29800.026.97450451
TTCTGCT30050.026.63528352
AGAAATG13200.025.87468769
TCTTCTG30450.025.832250
AATCTCG30500.025.78985236
TCTGCTT31000.025.59646653
CTGCTTG32700.025.42629854
CTCGTAT31200.024.99008239
TCTCGTA32700.023.84374838
Produced by FastQC (version 0.11.2)