FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2154011
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA76500.35515138966328397No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG57380.2663867547565913RNA PCR Primer, Index 17 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG24500.11374129472876415RNA PCR Primer, Index 17 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT21650.030.435744
TTTCGGA9150.030.182771
CCGTCTT20450.030.02856347
TCGTATG21900.029.9307340
GTATGCC22050.029.72712142
TGCCGTC21650.029.47960545
CTCGTAT20900.028.88681239
TATGCCG23050.028.73678243
GTCTTCT20950.027.99515249
GCCGTCT22100.027.94271346
CGTATGC23600.027.92088741
TGCTTGA22950.027.81038355
CTTGAAA24200.026.8009657
AGAAATG15650.026.67528769
TCTTCTG22350.026.39590350
CTTCTGC23050.025.89363951
CTGCTTG23800.025.65749454
GCTTGAA25500.025.29934356
TTCTGCT23800.025.07766352
TCTGCTT25550.023.63007253
Produced by FastQC (version 0.11.2)