FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80066
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG42825.348087827542278RNA PCR Primer, Index 46 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG19182.3955236929533137RNA PCR Primer, Index 46 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG18332.289361276946519RNA PCR Primer, Index 46 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG9511.1877700896760173RNA PCR Primer, Index 46 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG8871.1078360352709016RNA PCR Primer, Index 46 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGG3270.4084130592261384RNA PCR Primer, Index 46 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG2740.34221767042190193TruSeq Adapter, Index 4 (96% over 27bp)
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG2360.2947568256188644RNA PCR Primer, Index 46 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTATGCTTGAAAAAAAAAAAGGGG2320.28976094721854473Illumina PCR Primer Index 8 (96% over 26bp)
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG1520.18984337921214997TruSeq Adapter, Index 4 (96% over 27bp)
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG1200.1498763520095921RNA PCR Primer, Index 46 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGG1070.13363974720855296TruSeq Adapter, Index 4 (96% over 27bp)
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG990.12364799040791347RNA PCR Primer, Index 46 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG960.11990108160767367RNA PCR Primer, Index 46 (96% over 28bp)
TCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG870.10866035520695426TruSeq Adapter, Index 4 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTATGCTTGAAAAAAAAAAAGGGGGGG870.10866035520695426Illumina PCR Primer Index 8 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGGC253.0685205E-668.9987642
GGGTGAT150.002347698768.9987511
TCTCGGA150.002347698768.9987538
ACGTCTT208.4667394E-568.9987547
TAAGGGG150.002347698768.998758
GCTAAGG150.002347698768.998756
CTGTGTT150.002347698768.9987522
CATGGTT200.007334708751.7490655
CTGTCAC200.007334708751.749069
CCCTCTG200.007334708751.7490618
TTGTACG200.007334708751.7490638
CTAAGGG200.007334708751.749067
ACGGGTC200.007334708751.749068
TATGACG200.007334708751.7490643
CCCCCCT200.007334708751.7490615
CCCTTTC200.007334708751.7490638
GAGCATT200.007334708751.7490627
AAGGGGC200.007334708751.749069
ATCTGTT200.007334708751.7490665
CGTATGG352.2540518E-549.2848241
Produced by FastQC (version 0.11.2)