FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711547
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15990.22472162766479237No Hit
CTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATT8060.11327431638387907No Hit
ACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCCTCGCCACACGGG7720.10849599534535316No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATGA553.2545658E-431.3656482
ACGATTG500.00773320127.5998340
CTTTGCG500.00773320127.599839
CTACAAA1759.094947E-1225.633821
TACACCG700.001333395124.6427065
AGAAATG2400.024.43735169
CATGCTA1701.6734703E-1024.35279310
AGGACTA4000.024.1498535
ATCACTG4000.024.1498535
GTATATG1151.6931117E-624.0049131
ACAAAGG2450.023.940313
CGGTCCA2800.023.4105710
GCAACGG3850.023.2985620
CGGGTTA750.001992351122.9998615
ACTGTAC4200.022.9998598
CAATACG1653.1395757E-922.99985928
TCCATCA4100.022.720972
TGCAACG3800.022.6972319
GACCGTT2300.022.4998637
CCATCAC4300.022.4665583
Produced by FastQC (version 0.11.2)