FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202242
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG121921.0141053132397637TruSeq Adapter, Index 11 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG63400.5273480713533548TruSeq Adapter, Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG32910.2737385651141783TruSeq Adapter, Index 11 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG32660.27165911688328975RNA PCR Primer, Index 11 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25640.2132682105599372No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG18620.15487730423658466TruSeq Adapter, Index 11 (96% over 28bp)
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC12120.10081165023348045No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC43200.031.304842
GCTTGAA43200.031.06522256
ATGCCGT43600.031.017644
TATGCCG43800.030.95473343
GCCGTCT43550.030.89477746
TGCCGTC43750.030.75354445
CCGTCTT44300.030.60535647
CTTGAAA44050.030.4657857
TCTTCTG44100.030.4312450
CTTCTGC44300.030.37172751
CGTCTTC44350.030.259748
CTCGTAT43000.029.8457939
GTCTTCT45400.029.6358549
CGTATGC45850.029.57071541
TGCTTGA45750.029.40912655
TGAAAAA46550.029.27426759
CTGCTTG46150.029.15422854
TTGAAAA46400.028.99714758
TCTCGTA42750.028.89053238
TTTCGGA5250.027.6096691
Produced by FastQC (version 0.11.2)