FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894459
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41070.45916022981489374No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG39360.4400425285004679TruSeq Adapter, Index 11 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG20400.22807082269841322TruSeq Adapter, Index 11 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTCT14050.034.868149
CGATTAG400.002596110334.4997848
TGCCGTC14500.034.0239245
CCGTCTT14500.033.78598847
ATGCCGT14550.033.43277444
TATGCCG14850.032.75736243
GTATGCC14950.032.53825442
TCTCGTA14700.032.38754738
TCTTCTG15250.031.89815350
AATCGTA1305.456968E-1231.84594767
CTTCTGC15200.031.7761151
CTCGTAT15450.031.48523139
CTTGAAA15300.030.89195857
GCTTGAA15450.030.81533256
TGCTTGA15450.030.59203355
GCCGTCT15800.030.56942446
CGTATGC16550.030.0179341
CTGCTTG16000.029.54043454
CGTCTTC16700.028.92196748
TTCTGCT16350.028.9080752
Produced by FastQC (version 0.11.2)